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MR. NEMWEL OMWENGA NYAMWEYA

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Staff Information
PERSONAL DETAILS
Designation
SENIOR TECHNOLOGIST I
Corporate Email
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Job Category
Non-Teaching
Division / Faculty
Faculty of Science
Directorate / Dept
DEPT. OF BIOCHEMISTRY & MOLECULAR BIO
Main Campus Njoro
Address
P.o.Box 536 Egerton
ACADEMIC QUALIFICATIONS

2002 to 2003: Higher National Diploma (HND) Applied Biology, Technical University of Kenya; then The Kenya Polytechnic 

1999 to 2000: Ordinary National Diploma (OND) Applied Biology, Technical University of Kenya; then The Kenya Polytechnic 

1991 to 1995: Kenya Certificate of Secondary Education

1983 to 1990 :Kenya certificate of Primary Education

FIELDS OF SPECIALIZATION

Specialized Molecular Biology techniques like nucleic acid extractions, amplification by PCR, electrophoresis as well as optimization and troubleshooting.

PROFESSIONAL QUALIFICATIONS

March 2010: Workshop on specification writing in connection with procurement held at Egerton University in conjunction with The Kenya Bureau of Standards (KEBS).

November 2007: Training in continuous performance management at the International Livestock Research Institute (ILRI) in conjunction with independent Communication Associates (INCA)

October 2007: Workshop on HIV/AIDS training held at the United Nations (UN) in Nairobi

June 2006: Workshop on laboratory animal science at University of Nairobi in collaboration with the society of neuro-scientists and the division of comparative medicine University of Uppsla, Sweden.

March 2006: Radio-safety training held at International Livestock Research Institute (ILRI) in conjunction with the University of Nairobi.

WORK EXPERIENCE

2009 to Current

Egerton University, Department of Biochemistry & Molecular Biology

Molecular Biology practicals and General Biochemistry

  • Other responsibilities include the following:
    • In charge of the Molecular Biology laboratory.
    • Oversee day to day running and management of the laboratory.
    • Carry out and advice on various Molecular Biology techniques carried out in the lab by our regular students and others who would like to use the lab. These include: nucleic acid extractions, amplification by PCR, electrophoresis as well as optimization and troubleshooting.
    • Prepare, set up and conduct practicals for both undergraduate and postgraduate students.
    • Assist staff and students in their research and research projects respectively.
    •  Assist in the publication of research out puts.
    • Assist the Chief Technologist with day to day running of the department and act as the Chief Technologist when he is away or on leave.
    • Together with the chief technologist develop SOPs; ensure constant supply of consumables, reagents and equipment in the lab as well as their preventive maintenance.
  • Ensure quality assurance and quality control in the laboratory especially with safety and use of SOPs. Member of the quality assurance committee in the department.
    • Marking of practical reports for both undergraduate and postgraduate students.
    • Invigilating of examinations.

2001 to June 2009

International Livestock research Institute (ILRI)

  • Extraction (using various methods), standardization, cataloging and storage of DNA from various sources.
  • DNA amplification  (PCR). 
  • Extraction of high quality total RNA from various tissues for use in Micro-array work.
  • cDNA synthesis and subsequent use for qPCR assays
  • Preparing, running and processing both agarose and acryl amide gels.
  • Fragments analysis by genemapper software
  • Purification of monocytes and their subsequent cultivation as well as growth into macrophages and CD4 cells in the laboratory.
  • Purification of trypanosomes from blood and their subsequent cultivation and growth in the laboratory as well in laboratory animals
  • Challenging and subsequent monitoring of laboratory animals miceand cattle.
  • Train and assist visiting scientists, postgraduate students and undergraduate students on the various Molecular Biology techniques in the laboratory and in their research.
  • Liaise with the local area coordinator and manage SOPs, ensure constant supply of consumables, reagents and equipment in the lab as well as their preventive maintenance.
  • Ensure quality assurance and quality control in the laboratory especially with safety and use of SOPs.
  • Collect samples in the field and share them with our overseas collaborators.
  • Prepare serum and plasma and store it optimally for further analysis.
  • Microscopic examination of experimental animals using blood smears, prepare and read PCVs to ascertain levels of infection and enter this into databases.
  • Carry out basic analysis (using Microsoft excel) of phenotypic data from experimental animals especially survival data and parasitaemia.
LIST OF PUBLICATIONS
  1. Nyariki, J. N., Mokaya, D., Jillani, N. E., Nyamweya, N. O., Hoerauf, A., & Orina, A. (2019). Coenzyme Q10 Protect Mice Against Inflammatory Responses During Experimental Cerebral Malaria. Journal of Natural Sciences Research, 9(2), 43–51. https://doi.org/10.7176/jnsr/9-2-05
  2. Nyariki, J. N., Mokaya, D., Jillani, N. E., Nyamweya, N. O., & Isaac, A. O. (2019). Oral Administration of Coenzyme Q10 has the Capacity to Stimulate Innate Lymphoid Cells Class Two during Experimental Cerebral Malaria. 2(2), 1–9. https://doi.org/10.9734/SAJP/2019/46442
  3. Nyariki, J. N., Ochola, L. A., Jillani, N. E., Nyamweya, N. O., Amwayi, P. E., Yole, D. S., … Isaac, A. O. (2019). Oral administration of Coenzyme Q 10 protects mice against oxidative stress and neuro-inflammation during experimental cerebral malaria. Parasitology International, 71(August 2018), 106–120. https://doi.org/10.1016/j.parint.2019.04.010
TEACHING AREAS
  • Conducting Practical classes in Molecular Biology
  • Conducting Practical classes under General Biochemistry
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