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John Masani Nduko, Ph.D.

 

Dr. John Masani Nduko, Ph.D.

Lecturer, Biotechnology and Microbiology, Department of Dairy and Food Science and Technology.

Faculty of Agriculture

P.O. Box 536-20115

Egerton, Kenya.

Mobile: +254 728 851448

Email: ikobeusy@yahoo.co.uk; This e-mail address is being protected from spambots. You need JavaScript enabled to view it

EDUCATION

Year

Degree/ certificate

Institution

2014

PhD, Biotechnology and Macromolecular Chemistry

Hokkaido University, Japan

2011

M. Eng, Biotechnology and Macromolecular Chemistry

Hokkaido University, Japan

2005

BSc. Food Science and Technology (1st class honours)

Egerton University, Kenya

1998

Kenya Certificate of Secondary Education

Menyenya High School, Kenya

1994

Kenya Certificate of Primary Education

Ikobe Primary School, Kenya

PROFESSIONAL QUALIFICATIONS

Dates/Year

Title of Certificate

Institution Awarding

February 2014

Research proposal writing skills

Egerton University, Kenya

March, 2012

Preparing Future Faculty Certificate

(An introduction to teaching and writing)

Hokkaido University, Japan in collaboration with University of California, Berkeley, USA.

Oct 2008 – March 2009

Intensive course in Japanese language

International student centre, Hokkaido University, Japan

FIELD OF SPECIALIZATION

Food Science, Microbial Biotechnology, Microbiology, Protein Engineering, Polymer Chemistry, Metabolic Engineering, Synthetic Biology, Molecular Biology and Genetic Engineering

WORK EXPERIENCE

Jan 2014 - Present

Lecturer, Dept. of Dairy and Food Science and Technology

Oct 08March 2009

Research fellow, Graduate School of Engineering, Hokkaido University, Japan

April 2007 – Sept 2009

Customer Advisor, Barclays Bank of Kenya. Gilgil Branch.

May 04Aug 2004

Intern, Kenya Bureau of Standards Laboratories, Nairobi

KEY RESEARCH PROJECTS (2011 - Present)

Project period

Research Topic

April 2011 Dec 2013

Production of lactate (LA)-based polymers from lignocellulose biomass. Donor: Global Centre of Excellence (GCOE), Hokkaido University, Japan; 2011 - 2012; 350,000 Yen (4400 USD). PI: Prof. Seiichi Taguchi

RECENT SELECTED PUBLICATIONS (2010 - Present)

1

John Masani Nduko, Ken’ichiro Matsumoto, Toshihiko Ooi and Seiichi Taguchi. (2014). Enhanced production of poly(lactate-co-3-hydroxybutyrate) from xylose in engineered Escherichia coli overexpressing a galactitol transporter. Appl. Microbiol. Biotechnol. 98(6), 2453 - 2460.

2

Matsumoto Ken'ichiro, Terai Satsuki, Ishiyama Ayako, Sun Jian, Kabe Taizo, Song Yuyang, John Masani Nduko, Iwata Tadahisa, Taguchi Seiichi (2013). One-pot microbial production, mechanical properties and enzymatic degradation of isotactic P[(R)-2-hydroxybutyrate] and its copolymer with (R)-lactate. Biomacromolecules. 14 (6), 1913-1918.

3

John Masani Nduko, Ken’ichiro Matsumoto, Toshihiko Ooi and Seiichi Taguchi. (2013). Effectiveness of xylose utilization for high yield production of lactate-enriched P(lactate-co-3-hydroxybutyrate) using lactate-overproducing strain of Escherichia coli and evolved lactate-polymerizing enzyme. Metab. Eng. 15, 159-166.

4

Ken’ ichiro Matsumoto, John Masani Nduko and Seiichi Taguchi. (2013). Metabolic chemistry of microbial production of lactic acid and lactate-based polyesters (Article in Japanese). Chemistry and Biochemistry. Vol. 51, No. 7, pp. 448 – 456

5

John Masani Nduko, Suzuki Wakako, Ken’ichiro Matsumoto, Hirokazu Kobayashi, Toshihiko Ooi, Atsushi Fukuoka & Seiichi Taguchi. (2012). Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance to 5-hydroxymethylfurfural. J Biosci Bioeng, 113, 70-72.

6

Matsumoto, K., Mukai, Y., Ogata, D., Shozui, F., Nduko, J. M., Taguchi, S. and Ooi, T. (2010). Characterization of thermostable FMN-dependent NADH azoreductase from the moderate thermophile Geobacillus stearothermophilus. Appl. Microbiol. Biotechnol. 86, 1431 - 1438

7

John Masani Nduko, Ken’ ichiro Matsumoto and Seiichi Taguchi. Book title: Biobased monomers, polymers, and materials. Chapter title: Biological lactate-polymers synthesized by one-pot microbial factory: Enzyme and Metabolic Engineering. 2012. ACS Symposium Series Vol. 1105, Edited by Patrick B. Smith, Richard A. Gross. American Chemical Society, pp. 215 – 235. ISBN-13: 9780841227675

8

John Masani Nduko, Ken’ichiro Matsumoto and Seiichi Taguchi. Book title: Green Polymer Chemistry: Biocatalysis and Materials II. Chapter title: Microbial Plastic Factory: Synthesis and properties of the new lactate-based and related biopolymers. 2013. ACS Symposium Series, Vol. 1144, edited by H. N. Cheng, R. Gross and P. Smith. American Chemical Society, pp. 175 – 197. ISBN13: 9780841228955. eISBN: 9780841228962

9

Yuyang Song, John Masani Nduko, Ken’ichiro Matsumoto, Seiichi Taguchi. Microbial factory for the production of polyesters: a new platform of Corynebacterium glutamicum. Book title: Corynebacterium glutamicum: Systems Biology, Biotechnological Applications and Control. Caister Academic Press, 2014. (In press).

SELECTED CONFERENCE PRESENTATIONS (2010 - present)

1.

John Masani Nduko, Ken’ichiro Matsumoto, Toshihiko Ooi, and Seiichi Taguchi. Efficient production of poly(lactate-co-hydroxybutyrate)using hemicelluloses derived sugar, xylose, in engineered Escherichia coli overexpressing a galactitol transporter. Frontier Chemistry center, The 2nd International symposium (Advanced Materials Science) (9 – 10 Dec, 2013), Sapporo, Japan.

2.

Jian Sun, Ken’ichiro Matsumoto, John Masani Nduko and Seiichi Taguchi. Biosynthesis, Physical properties and enzymatic degradation of isotactic (R)-2-hydroxybutyrate-based polyester. Frontier Chemistry center, The 2nd International symposium (Advanced Materials Science) (9 – 10 Dec, 2013), Sapporo, Japan.

3.

Toshihiko Ooi, Sasaki Shouhei, John Nduko, Ken’ ichiro Matsumoto and Seiichi Taguchi. Microbial production of P(3HB) from plant biomass. The Society for biotechnology of Japan, 65th meeting. September 18 - 20th 2013. Hiroshima, Japan.

4.

Shouhei Sasaki, Toshihiko Ooi, John Nduko, Ken’ ichiro Matsumoto and Seiichi Taguchi. Copolymer production from plant biomass using recombinant E. coli. The Society for Biotechnology of Japan, 65th meeting. September 18 - 20th 2013. Hiroshima, Japan.

5.

Shouhei Sasaki, Nduko, J M, Ken’ ichiro Matsumoto, Toshihiko Ooi and Seiichi Taguchi. Efficient production of lactate-enriched lactate-based polymers from lignocellulosic biomass. The Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA), Hokkaido branch, November, 2012. Hokkaido University, Japan.

6.

S. Taguchi, K. Matsumoto, M. Yamada, Y. Song, J. Nduko, Microbial plastic factory: ‘New lactate-based and related biopolymers’ (Invited Lecture), International Conference on Bioinspired and Biobased Chemistry & Materials. October 3- 5th, 2012. Nice, France.

7.

John Masani Nduko, Ken’ ichiro Matsumoto, Toshihiko Ooi and Seiichi Taguchi. Efficient bioconversion of lignocellulosic biomass-derived sugars into poly[lactate-co-3-hydroxybutyrate) by metabolically engineered Escherichia coli, The 15th International Biotechnology Symposium (IBS 2012). September 16 - 21st 2012. EXCO, Daegu, South Korea.

8.

John M Nduko, Ken' ichiro Matsumoto, Yuyang Song, Seiichi Taguchi. Microbial plastic factory: Synthesis and properties of the new lactate-based and related biopolymers, The 244th American Chemical Society (ACS) National Meeting & Exposition (August 19 - 23rd, 2012), Philadelphia, Pennsylvania, USA.

9.

Nduko John Masani, Ken’ ichiro Matsumoto and Seiichi Taguchi. Biosynthesis and properties of advanced biopolymers containing lactate by metabolically engineered bacteria, The 244th American Chemical Society (ACS) National Meeting & Exposition (August 19 - 23rd, 2012), Philadelphia, Pennsylvania, USA.

10.

John Masani Nduko, Ken’ ichiro Matsumoto, Toshihiko Ooi and Seiichi Taguchi. Lactate-based polyesters production by recombinant bacteria using lignocellulosic biomass sugars as carbon sources, The 12th Japan-China-Korea Joint Symposium on Enzyme Engineering (May 28 - 31st 2012), Kanazawa, Japan

11.

John Masani Nduko, Ken’ ichiro Matsumoto, Toshihiko Ooi and Seiichi Taguchi. Bioconversion of the hemicellulosic sugar, xylose, into lactate-based polyesters using recombinant Escherichia coli, The American Chemical Society (ACS), 243rd National Meeting and Exposition (March 25 - 29th, 2012), San Diego, California-USA

12.

John Masani Nduko, Ken’ichiro Matsumoto, Hirokazu Kobayashi, Toshihiko Ooi, Atsushi Fukuoka and Seiichi Taguchi. Biosynthesis of polyhydroxyalkanoates from cellulose hydrolysate in Escherichia coli strain with superior tolerance to 5-hydroxymethylfurfural, The 5th GCOE international symposium, February 21 - 22nd, 2012. Conference hall, Hokkaido University, Sapporo, Japan

13.

John Masani Nduko, Ken’ichiro Matsumoto, Toshihiko Ooi, and Seiichi Taguchi. Production of lactate-based polyesters from xylose in Escherichia coli, The Society for Biotechnology, Japan. 63rd Annual meeting (September 26 - 28th, 2011). Tokyo University of Agriculture and Technology, Japan.

14.

John Masani Nduko, Wakako Suzuki, Ken’ichiro Matsumoto, Hirokazu Kobayashi, Toshihiko Ooi, Atsushi Fukuoka and Seiichi Taguchi. Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance to 5-hydroxymethylfurfural, The 2011 Taiwan-Japan bilateral polymer symposium, September 14 – 17th, 2011. National Chiao Tung University, Hsinchu, Taiwan

15.

Nduko John Masani, Ken’ ichiro Matsumoto and Seiichi Taguchi. Efficient xylose utilization in microbial production of lactate (LA)-based polymers. The 45th Society of Polymer Science Japan, Hokkaido branch, February 1st, 2011. Hokkaido University, Japan.

16.

J. Nduko, D. Ogata, T. Okei, K. Matsumoto, T. Ooi and Taguchi. S. Molecular cloning, purification and characterization of an azoreductase from psychrotolerant Exiguobacterium sibiricum, The Society for Biotechnology, Japan. July 23rd, 2010. Kitami Institute of Technology, Hokkaido, Japan

AWARDS

Year

Organization

2008- 2014

Research fellow, Masters and Doctors courses Scholarship by the Japanese government

2012

Travel award (150 000 yen). Award to attend the 15th International Biotechnology Symposium in Daegu, Korea. September, 2012. Grant institution; Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, Japan.

2011

Professor Otsuka Hiroshi memorial prize. Awarded for an outstanding record in the Masters course at the Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Japan.

MEMBERSHIP TO PROFESSIONAL / SCIENTIFIC ASSOCIATIONS

Membership Category

Organization

Member

American Chemical Society (ACS)

Member

The Society for Biotechnology, Japan